The fact is that formalin-fixed tissue needs an antigen retrieval procedure before immunohistochemical staining can begin. This is because of the formation of methylene bridges that occurs during fixation. It takeovers link proteins and thus mask antigenic sites. There are two methods recommended for Antigen Retrieval - enzymatic or heat mediated. Both of these Antigen Retrieval Methods will assist to break the methylene bridges in order to expose the sites to endow the antibodies to bind.
Moreover, the enzymatic method is considered as a gentler process than heat mediated method. Thus, it is well recommended for sensitive tissues. It should be noted that the enzymatic method takes much time than the heat mediated method. But this method is quite technically demanding. Moreover, in a case, the antigen retrieval step is stated on the antibody datasheet, start someone by trying the heat mediated method. In a case, you will not succeed, then again try with the enzymatic method.
Let’s comprehensively discuss both processes of Antigen Retrieval Method
In this, tissues procedure is done in the best way on amino-propyl-tri-ethoxy-silane coated slides. Moreover, these should be placed in a standard rack for this process.
For this Antigen Retrieval Method, there will be no use of the domestic microwave. Only a scientific microwave is recommended for this.
Booster Antibody and Elisa experts is a prominent destination to get more guidance about Antigen Retrieval Methods. To contact Booster Antibody and Elisa experts visit our website: https://immunostaining.info/
Founded in 1993 by famed histologist Steven Xia, Boster Bio is an antibody manufacturer specializing in high-sensitivity, high-specificity, ELISA kits and WB/IHC compatible antibodies.